主题：【合作】玉米种子 PH4CV专利翻译合作 -- 急风劲草

通过SSR的基因标记物(Genetic Marker Profile through SSR)

The present invention comprises an inbred corn plant which is characterized by the molecular and physiological data presented herein and in the representative sample of said line deposited with the ATCC. Further provided by the invention is a hybrid corn plant formed by the combination of the disclosed inbred corn plant or plant cell with another corn plant or cell and characterized by being heterozygous for the molecular data of the inbred.

In addition to phenotypic observations, a plant can also be identified by its genotype. The genotype of a plant can be characterized through a genetic marker profile which can identify plants of the same variety or a related variety or be used to determine or validate a pedigree. Genetic marker profiles can be obtained by techniques such as Restriction Fragment Length Polymorphisms (RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs), Arbitrarily Primed Polymerase Chain Reaction (AP-PCR), DNA Amplification Fingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) which are also referred to as Microsatellites, and Single Nucleotide Polymorphisms (SNPs). For example, see Berry, Don, et al., “Assessing Probability of Ancestry Using Simple Sequence Repeat Profiles: Applications to Maize Hybrids and Inbreds”, Genetics, 2002, 161:813-824, which is incorporated by reference herein in its entirety.

Particular markers used for these purposes are not limited to the set of markers disclosed herein, but are envisioned to include any type of marker and marker profile which provides a means of distinguishing varieties. In addition to being used for identification of Inbred Line PH4CV, a hybrid produced through the use of PH4CV, and the identification or verification of pedigree for progeny plants produced through the use of PH4CV, the genetic marker profile is also useful in breeding and developing single gene conversions.

Means of performing genetic marker profiles using SSR polymorphisms are well known in the art. SSRs are genetic markers based on polymorphisms in repeated nucleotide sequences, such as microsatellites. A marker system based on SSRs can be highly informative in linkage analysis relative to other marker systems in that multiple alleles may be present. Another advantage of this type of marker is that, through use of flanking primers, detection of SSRs can be achieved, for example, by the polymerase chain reaction (PCR), thereby eliminating the need for labor-intensive Southern hybridization. The PCR detection is done by use of two oligonucleotide primers flanking the polymorphic segment of repetitive DNA. Repeated cycles of heat denaturation of the DNA followed by annealing of the primers to their complementary sequences at low temperatures, and extension of the annealed primers with DNA polymerase, comprise the major part of the methodology.

Following amplification, markers can be scored by gel electrophoresis of the amplification products. Scoring of marker genotype is based on the size of the amplified fragment as measured by molecular weight (MW) rounded to the nearest integer. While variation in the primer used or in laboratory procedures can affect the reported molecular weight, relative values should remain constant regardless of the specific primer or laboratory used. When comparing lines it is preferable if all SSR profiles are performed in the same lab. The SSR analyses reported herein were conducted in-house at Pioneer Hi-Bred. An SSR service is available to the public on a contractual basis by Paragen (formerly Celera AgGen) in Research Triangle Park, N.C.

Primers used for the SSRs reported herein are publicly available and may be found at the World Wide Web at agron.missouri.edu/maps.html (sponsored by the University of Missouri), in Sharopova et al. (Plant Mol. Biol. 48(5-6):463-481), Lee et al (Plant Mol. Biol. 48(5-6); 453-461), or reported herein. Some marker information may be available from Paragen.

Map information is provided in centimorgans (cM) and based on a composite map developed by Pioneer Hi-Bred. This composite map was created by identifying common markers between various maps and using linear regression to place the intermediate markers. The reference map used was UMC98. Map positions for the SSR markers reported herein will vary depending on the mapping population used. Any chromosome numbers reported in parenthesis represent other chromosome locations for such marker that have been reported in the literature or on the Maize DB. Map positions are available on the Maize DB for a variety of different mapping populations.

The SSR profile of Inbred PH4CV can be used to identify hybrids comprising PH4CV as a parent, since such hybrids will comprise the same alleles as PH4CV. Because an inbred is essentially homozygous at all relevant loci, an inbred should, in almost all cases, have only one allele at each locus. In contrast, a genetic marker profile of a hybrid should be the sum of those parents, e.g., if one inbred parent had the allele 168 (base pairs) at a particular locus, and the other inbred parent had 172 the hybrid is 168.172 (heterozygous) by inference. Subsequent generations of progeny produced by selection and breeding are expected to be of genotype 168 (homozygous), 172 (homozygous), or 168.172 for that locus position. When the F1 plant is used to produce an inbred, the locus should be either 168 or 172 for that position.

In addition, plants and plant parts substantially benefiting from the use of PH4CV in their development such as PH4CV comprising a single gene conversion, transgene, or genetic sterility factor, may be identified by having a molecular marker profile with a high percent identity to PH4CV. Such a percent identity might be 98%, 99%, 99.5% or 99.9% identical to PH4CV.

The SSR profile of PH4CV also can be used to identify essentially derived varieties and other progeny lines developed from the use of PH4CV, as well as cells and other plant parts thereof. Progeny plants and plant parts produced using PH4CV may be identified by having a molecular marker profile of at least 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 99.5% genetic contribution from inbred line PH4CV.

种子储蓄(Deposits)

Applicant has made a deposit of at least 2500 seeds of Inbred Maize Line PH4CV with the American Type Culture Collection (ATCC), Manassas, Va. 20110 USA, ATCC Deposit No. PTA-4673. The seeds deposited with the ATCC on Sep. 18, 2002 were taken from the deposit maintained by Pioneer Hi-Bred International, Inc., 800 Capital Square, 400 Locust Street, Des Moines, Iowa 50309-2340 since prior to the filing date of this application. Access to this deposit will be available during the pendency of the application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request. Upon allowance of any claims in the application, the Applicant will make the deposit available to the public pursuant to 37 C.F.R. § 1.808. This deposit of the Inbred Maize Line PH4CV will be maintained in the ATCC depository, which is a public depository, for a period of 30 years, or 5 years after the most recent request, or for the enforceable life of the patent, whichever is longer, and will be replaced if it becomes nonviable during that period. Additionally, Applicant has satisfied all the requirements of 37 C.F.R. §§1.801-1.809, including providing an indication of the viability of the sample upon deposit. Applicant has no authority to waive any restrictions imposed by law on the transfer of biological material or its transportation in commerce. Applicant does not waive any infringement of his rights granted under this patent or under the Plant Variety Protection Act (7 USC 2321 et seq.). U.S. Plant Variety Protection of Inbred Maize Line PH4CV has been applied for under Application No. 200200176.

All publications, patents and patent applications mentioned in the specification are indicative of the level of those skilled in the art to which this invention pertains. All such publications, patents and patent applications are incorporated by reference herein to the same extent as if each was specifically and individually indicated to be incorporated by reference herein.

The foregoing invention has been described in detail by way of illustration and example for purposes of clarity and understanding. However, it will be obvious that certain changes and modifications such as single gene conversions and mutations, somocional variants, variant individuals selected from large populations of the plants of the instant inbred and the like may be practiced within the scope of the Invention, as limited only by the scope of the appended claims.

Claims:

权利要求:

1. A seed of maize inbred line designated PH4CV, representative seed of said line having been deposited under ATCC Accession No. PTA-4673.

1. 一个指定为PH4CV的玉米自交系种子，此玉米自交系的代表种子已被储存在美国标准生物品收藏中心登录号PTA – 4673下。

2. A maize plant, or a part thereof, produced by growing the seed of claim 1.

2. 通过种植权利要求1中的种子得到的一个玉米植株，或玉米植株的一个组成部分。

3. The maize plant of claim 2, wherein said plant has been detasseled.

3. 权利要求2中的, 已经被去顶端雄穗, 的玉米植株.

4. A tissue culture of regenerable cells produced from the plant of claim 2.

4. 由权利要求2中的植株得到的可再生细胞的组织培养基。

5. A protoplast produced from the tissue culture of claim 4.

5. 由权利要求4中的组织培养基得到的原生质体。

6. The tissue culture of claim 4, wherein cells of the tissue culture are produced from a tissue selected from the group consisting of leaf, pollen, embryo, root, root tip, anther, silk, flower, kernel, ear, cob, husk and stalk.

6. 权利要求4中的组织培养基，其中组织培养的细胞是来源于下列植物组织之一，包括叶，花粉，胚，根，根尖，花药，丝穗，花，核，玉米穗，穗轴，稻壳和秸秆.

7. A maize plant regenerated from the tissue culture of claim 4, said plant having all the morphological and physiological characteristics of inbred line PH4CV, representative seed of said inbred line having been deposited under ATCC Accession No. PTA-4673.

7. 从权利要求4的组织培养基再生成的一个玉米植株，所说的植株具有自交系PH4CV所有的种子形态和生理特性. 此自交系PH4CV的代表种子，已被储备在美国标准生物品收藏中心登录号PTA- 4673下.

8. A method for producing an F1 hybrid maize seed, comprising crossing the plant of claim 2 with a different maize plant and harvesting the resultant F1 hybrid maze seed.

8. 一个产生F1杂交玉米种子的方法，其中包括将权利要求2中得到的玉米植株与另一个不同的玉米植株互交, 产生的F1杂交玉米种子。

9. A method of producing a male sterile maize plant comprising transforming the maize plant of claim 2 with a nucleic acid molecule that confers male sterility.

9. 一个产生雄性不育玉米植株的方法, 此雄性不育玉米植株是改变权利要求2中的玉米植株, 使之被赋予雄性不育的核酸分子。

10. A male sterile maize plant produced by the method of claim 9.

10. 由权利要求9中的方法产生的雄性不育玉米植物。

11. A method of producing an herbicide resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers herbicide resistance.

11. 产生一种抗除草剂玉米植株的方法, 这种植株是把权利要求2中的玉米转换使之带有抗除草剂的转基因。

12. An herbicide resistant maize plant produced by the method of claim 11.

12. 由权利要求11中的方法所产生的, 一种抗除草剂玉米植株。

13. The maize plant of claim 12, wherein the transgene confers resistance to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.

13. 权利要求12中的玉米植株，其中转基因具有抵抗来自下列一组除草剂中的一种: 咪唑啉，磺酰脲类，草甘膦，草铵膦，L型膦，三嗪类和腈。

14. A method of producing an insect resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers insect resistance.

14. 一个产生抗虫玉米的植物的方法，包括改造权利要求2中的植株，使之具有抗虫转基因。

15. An insect resistant maize plant produced by the method of claim 14.

15. 一种由权利要求14中的方法所产生的抗虫玉米植株。

16. The maize plant of claim 15, wherein the transgene encodes a Bacillus thuringiensis endotoxin.

16. 权利要求15中的玉米植株，其转基因编码一种苏云金芽孢杆菌内毒素。

17. A method of producing a disease resistant maize plant comprising transforming the maize plant of claim 2 with a transgene that confers disease resistance.

17. 一个产生抗病玉米植物的方法，此方法改造权利要求2中的植株，使之具有抗病转基因。

18. A disease resistant maize plant produced by the method of claim 17.

18. 由权利要求17产生的一种抗疾病的玉米植株.

19. A method of producing a maize plant with decreased phytate content comprising transforming the maize plant of claim 2 with a transgene encoding phytase.

19. 一个生产含有低量植酸含量的玉米植株的方法，包括转化权利要求2中的玉米植株, 使之带有编码植酸酶的转基因.

20. A maize plant with decreased phytate content produced by the method of claim 19.

20. 由权利要求19的方法产生的一个含有低量植酸含量的玉米植株.

21. A method of producing a maize plant with modified fatty acid metabolism or modified carbohydrate metabolism comprising transforming the maize plant of claim 2 with a transgene encoding a protein selected from the group consisting of fructosyltransferase, levansucrase, alpha-amylase, invertase and starch branching enzyme or encoding an antisense of stearyl-ACP desaturase.

21. 一种方法能产生脂肪酸代谢得到改变, 或者糖代谢得到改变的玉米植株, 此方法包括改变权利要求2中的玉米植株, 使之带有编码一种蛋白质的转基因, 这种蛋白质选自, 包括果糖转移酶，果聚糖生成酶，α-淀粉酶，蔗糖酶和淀粉分支酶或者编码硬脂-ACP去饱和酶的一个反义蛋白。

22. A maize plant, with modified fatty acid metabolism or modified carbohydrate metabolism, produced by the method of claim 21.

22. 由权利要求21中的方法生产出的一个脂肪酸代谢或者碳水化合物代谢得到改良或者修饰的玉米植物。

23. The maize plant of 22 wherein the transgene confers a trait selected from the group consisting of waxy starch and increased amylose starch.

23. 22中的玉米植株，其转基因带有选自从蜡质淀粉和直链增加淀粉的一个特点。

24. A maize plant, or part thereof, having all the physiological and morphological characteristics of the inbred line PH4CV, representative seed of said inbred line having been deposited under ATCC Accession No. PTA-4673.

24。一个玉米植株，或其中一个组成部分，具有自交系PH4CV所有的形态和生理特性，此自交系的代表种子已被存放于登录号是PTA- 4673的美国标准生物品收藏中心。

25. A method of introducing a desired trait into maize inbred line PH4CV comprising: (a) crossing PH4CV plants grown from PH4CV seed, representative seed of which has been deposited under ATCC Accession No. PTA-4673, with plants of another maize line that comprise a desired trait to produce F1 progeny plants, wherein the desired trait is selected from the group consisting of male sterility, herbicide resistance, insect resistance, disease resistance and waxy starch; (b) selecting F1 progeny plants that have the desired trait to produce selected F1 progeny plants; (c) crossing the selected progeny plants with the PH4CV plants to produce backcross progeny plants; (d) selecting for backcross progeny plants that have the desired trait and physiological and morphological characteristics of maize inbred line PH4CV listed in Table 1 to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of maize inbred line PH4CV listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

25。引入玉米自交系PH4CV所需的特征的一种方法，包括：

(a) 把PH4CV种子成长起来的PH4CV植物，其中代表性的种子已经储存在ATCC登录号PTA- 4673，与另一种具有所需特征的植物互交，产生F1后代植株, 其中所需的特征是从雄性不育，具除草剂抗性，抗虫性，抗病性和蜡质性淀粉组成的植株组中选择;

（b）选择具有所需植株性状的F1后代植株来产生选择性的F1后代植株;

（c）把被选择的后代植株与PH4CV植物选择回交后产生回交子代植株;

（d）选择在表1中列出的具有所需特质和生理与形态特性的玉米自交系PH4CV的回交子代植株, 以产生被选择的回交子代植株;

（e）重复步骤（c）和（d）连续三次或更多次, 以产生第四或更高代的回交后代植株, 在同样的生长环境条件下, 与5％显著性水平, 这些植株具有所需的特质和表1所列的玉米自交系PH4CV所有的生理和形态特征。

26. A plant produced by the method of claim 25, wherein the plant has the desired trait and all of the physiological and morphological characteristics of maize inbred line PH4CV listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

26. 由权利要求25中的方法产生的植物，其中，在同样的生长环境条件下, 与5％显著性水平, 该植株具有所需的特质和表1所列的玉米自交系PH4CV所有的生理和形态特征。

27. The plant of claim 26 wherein the desired trait is herbicide resistance and the resistance is conferred to an herbicide selected from the group consisting of imidazolinone, sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine and benzonitrile.

27. 权利要求26中产生的植株，其中的特点是抗除草剂, 这种耐受是指对来自咪唑啉，磺酰脲类，草甘膦，草铵膦，L型膦，三嗪类和腈, 中的一种除草剂的耐受。

28. The plant of claim 26 wherein the desired trait is insect resistance and the insect resistance is conferred by a transgene encoding a Bacillus thuringiensis endotoxin.

28. 权利要求26中的植株，其中所需的特质是抗虫性, 这种抗虫性来自编码苏云金杆菌内毒素的转基因。

29. The plant of claim 26 wherein the desired trait is male sterility and the trait is conferred by a cytoplasmic nucleic acid molecule that confers male sterility.

29. 权利要求26中的植株，其中所需的特质是雄性不育, 这种性状是由能带来雄性不育的细胞质的核酸分子赋予的。

30. A method of modifying fatty acid metabolism, phytic acid metabolism or carbohydrate in maize inbred line PH4CV comprising: (a) crossing PH4CV plants grown from PH4CV seed, representative seed of which has been deposited under ATCC Accession No. PTA-4673, with plants of another maize line that comprise a nucleic acid molecule encoding an enzyme selected from the group consisting of phytase, fructosyltransferase, levansucrase, alpha-amylase, invertase and starch branching enzyme or encoding an antisense of stearyl-ACP desaturase; (b) selecting F1 progeny plants that have said nucleic acid molecule to produce selected F1 progeny plants; (c) crossing the selected progeny plants with the PH4CV plants to produce backcross progeny plants; (d) selecting for backcross progeny plants that have said nucleic acid molecule and physiological and morphological characteristics of maize inbred line PH4CV listed in Table 1 to produce selected backcross progeny plants; and (e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny plants that comprise said nucleic acid molecule and have all of the physiological and morphological characteristics of maize inbred line PH4CV listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

30. 一种用于改变玉米自交系PH4CV的脂肪酸代谢，植酸代谢, 或糖代谢的方法, 包括：

（a）把PH4CV种子成长起来的PH4CV植物，其中代表性的种子已经储存在ATCC登录号PTA- 4673下，与另一种玉米的植株互交，此另一种玉米线核酸分子编码来自下列一组酶中的某一种, 包括植酸酶，果糖转移酶，果聚糖生成酶，α-淀粉酶，蔗糖酶和淀粉分支酶或者编码硬脂-ACP去饱和酶的一个反义蛋白;

（b）选择具有所说核酸分子的F1后代植株来产生选择性的F1后代植株;

（c）把被选择的后代植株与PH4CV植物选择回交后产生回交子代植株;

（d）选择在表1中列出的具有所说核酸分子和生理与形态特性的玉米自交系PH4CV的回交子代植株, 以产生被选择的回交子代植株;

（e）重复步骤（c）和（d）连续三次或更多次, 以产生第四或更高代的回交后代植株, 在同样的生长环境条件下, 与5％显著性水平, 这些植株具有所说核酸分子和表1所列的玉米自交系PH4CV所有的生理和形态特征。

31. A plant, with modified fatty acid metabolism, modified phytic acid metabolism, or modified carbohydrate metabolism, produced by the method of claim 30, wherein the plant comprises the nucleic acid molecule and has all of the physiological and morphological characteristics of maize inbred line PH4CV listed in Table 1 as determined at the 5% significance level when grown in the same environmental conditions.

31. 由权利要求30的方法产生的, 具有改变的脂肪酸代谢，改变的植酸代谢，改变的糖代谢, 的一个植株, 其中，在同样的生长环境条件下, 与5％显著性水平, 该植株具有所说核酸分子和表1所列的玉米自交系PH4CV所有的生理和形态特征。

• 【合作】玉米种子 PH4CV专利翻译合作

对于需要集体协作的翻译项目，似可先建立术语规范，可以通过楼主协调或共享专用ID的办法来解决。如果术语较多，也可以分成A-G、H-N等贴。这样可以把术语统一起来，方便合作。

协作机制已经说了，可以通过私信来协调或者共享ID在一个帖子里交流，认领任务。

外链出处

我国部分地区大老鼠绝迹 被指与转基因玉米有关

外链出处

不知道说什么好，只能送花、宝推了。

唯一的遗憾是我不懂生物，不能贡献自己的力量。

不知道说什么好，只能送花、宝推了。

唯一的遗憾是我不懂生物，不能贡献自己的力量。