主题：超精细光学显微镜 -- chela

衍射极限的技术看来发展前途远大，很有潜力。

Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission

Thomas A. Klar, Stefan Jakobs, Marcus Dyba, Alexander Egner, and Stefan W. Hell†

Max-Planck-Institute for Biophysical Chemistry, High Resolution Optical Microscopy Group, 37070 Göttingen, Germany

Edited by Daniel S. Chemla, E. O. Lawrence Berkeley National Laboratory, Berkeley, CA, and approved May 12, 2000

Received March 10, 2000.

Abstract

The diffraction barrier responsible for a finite focal spot size and limited resolution in far-field fluorescence microscopy has been fundamentally broken. This is accomplished by quenching excited organic molecules at the rim of the focal spot through stimulated emission. Along the optic axis, the spot size was reduced by up to 6 times beyond the diffraction barrier. The simultaneous 2-fold improvement in the radial direction rendered a nearly spherical fluorescence spot with a diameter of 90–110 nm. The spot volume of down to 0.67 attoliters is 18 times smaller than that of confocal microscopy, thus making our results also relevant to three-dimensional photochemistry and single molecule spectroscopy. Images of live cells reveal greater details.