主题：最近对各国政治领导力的一点感想 -- 仲明

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我用鼠疫当关键词，局限于2022年，学科范围是流行病/预防医学、微生物学，搜索到几十篇文章。下面例子是今年四月份的。你看看是不是生物武器。作者是不是没有经费支持，自费献爱心的。

A Novel Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Yersinia pestis

By:

Bai, Y (Bai, Ying) [1] ; Rizzo, MR (Rizzo, Maria Rosales) [1] ; Parise, C (Parise, Christina) [1] ; Maes, S (Maes, Sarah) [1] ; Eisen, RJ (Eisen, Rebecca J.) [1]

FRONTIERS IN MICROBIOLOGY

Volume13

Article Number863142

DOI10.3389/fmicb.2022.863142

PublishedAPR 7 2022

Indexed2022-05-15

Document TypeArticle

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Abstract

Rapid detection of Yersinia pestis, the causative agent of plague, is essential during field investigations to enable prompt control measures for prevention of the spread of the disease. Affordable, efficient, reliable, and simple detection assays are extremely useful, particularly in plague-endemic regions with limited resources. We developed a loop-mediated isothermal amplification (LAMP) assay that detects Y. pestis within 30 min by simply incubating at 65 degrees C on a dry bath heater. The assay targeted the caf1A gene that is situated on the pMT1 plasmid using six specific primers. Y. pestis presence is visually detected based on the color change in the reactions. For comparison of the assay performance, a real-time LAMP with fluorescent dye detection was conducted on a real-time PCR instrument using the same six primers. Sensitivity assessment showed that the limit of detection (LOD) was 0.2 and 0.03 pg when performed on the dry bath heater and on the real-time PCR instrument, respectively. The assay was 100% specific, having no cross-reactivity with closely related Yersinia spp. and other bacterial species. We tested the LAMP assay on field-collected fleas and showed that it successfully detected Y. pestis with identical results to that of a previously published pentaplex real-time PCR assay. These findings suggest that the relatively inexpensive and simpler LAMP assay could be used to support field investigations, yielding comparable results to more expensive and complex PCR assays.

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