主题：【整理】中国开发出替代CAS9的新型基因编程技术，诺奖级 -- 尖石
他还进一步说，原来的版本是初级版，实验手法比较复杂，需要较高的实验技巧，他又特别研发了升级版2.0版，以及smart版，据说很快会对社会公开。
今天我在知乎还看到一个印度实验室声称能够重复了。
1:17 PM (3 hours ago)
Hello everyone,
we have successfully used NgAgo in HeLa cells following a protocol where we used 700ng NgAgo plasmid and 500 ng gDNA (non purified, heat inactivated PNK). We confirmed knockout of GFP from a plasmid and could see the effect even after 96h. About 40% reduction in Western blot could be seen although there is scope for more optimization.
cheers
debo
作者：John Han
链接：https://www.zhihu.com/question/46968937/answer/108492103
No way to know yet actually since we have done this once just to check if there is a reduction in gfp which we observed both by facs and western. We are currently sequencing the products to rule out Rnai if any. Will let you know if that's confirmed
Best
Debo
Dr. Debojyoti Chakraborty
Room 129, CSIR-Institute of Genomics and Integrative Biology
Mathura Road,
New Delhi - 110025
- 相关回复 上下关系8
🙂国外60多家做了，20多重复了 4 混沌之源 字478 2016-06-29 15:51:15
🙂待认可未通过。偏要看
🙂学霸跟我有屁关系，我是真心希望这是个好技术，能跟 3 关中农民 字183 2016-06-29 16:47:27
🙂韩本人在北大演讲透露，有20多家能重复
🙂现在关键就在这个测序，所有号称能重复的目前 3 关中农民 字705 2016-06-30 10:16:56
🙂应该是DNA链的拓扑的问题 6 HAL9000 字434 2016-07-01 08:56:21
🙂我感觉这里有偷换概念，体外操作基因技术才是诺奖级 1 十二级 字972 2016-06-20 09:23:38
🙂这个对韩春雨的介绍很好，就是一个纯粹喜爱研究的人， 25 关中农民 字9337 2016-05-19 09:59:39